iBioTech

with New England Biolabs


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NEW PRODUCT:
HIFI TAQ DNA LIGASE
Optimized HiFi Taq DNA Ligase efficiently seals nicks in DNA with unmatched fidelity. Ligation of two adjacent oligonucleotides that are hybridized to a complementary target DNA is enhanced by an improved reaction buffer, and mismatch ligation is dramatically reduced. Furthermore, HiFi Taq DNA Ligase exhibits increased thermostability and is active at elevated temperatures (37-75°C) for extended cycles. This improved formulation allows for higher resolution discrimination between ligation donors and acceptors at both the 3′ and 5′side of the ligation junction, enabling precise detection of SNPs and other allele variants via detection methods such as LCR and LDR.
HiFi Taq DNA Ligase exhibits increased discrimination at both sides of the ligation junction
Oligonucleotide probes targeting a 35 bp region of the λ integrase gene were incubated with 16.7 fmol of λ genomic DNA. Ligation products formed were detected by qPCR using SYBR® Green. HiFi Taq DNA Ligase (NEB #M0647) displays increased fidelity over Taq DNA Ligase (NEB #M0208) and Ampligase®, showing a larger ΔCt value between probes fully complementary to the target sequence and those with a mismatched base pair at the ligation junction.
FEATURE ARTICLE:
SUBSTRATE SPECIFICITY AND MISMATCH DISCRIMINATION IN DNA LIGASES
DNA ligases vary in their ability to join fragments, add adaptors, repair nicks or breaks, link vectors and inserts, and to circularize dsDNA. Ligases also vary in their type of activity. The specificity and accuracy of the ligation depends upon ligase selection and careful optimization of reaction conditions. With the right ligase, conditions and probes, even single-base variations in sequence can be reliably detected.
Ligase Chain Reaction (LCR)
In this method, two pairs of probes are designed, one pair complementary to the top strand of the target, one pair to the bottom. Upon melting and annealing, both probe pairs can anneal to the target, and ligate efficiently if they form a nicked sequence without gaps or mismatches. On successive rounds of melting and re-annealing, unligated probe can now anneal to both the original target DNA and to the probes ligated in previous rounds. As each ligation product becomes a template for the complementary probe pair, LCR enables exponential amplification of the ligated product.

FEATURED PRODUCT:
PROTEIN STANDARDS

Suitable for analysis of a wide range of expressed proteins

  • Uniform band intensities
  • Convenient band spacing
  • Easy-to-identify reference bands
  • Prestained protein standards auto-fluoresce

GET FREE POSTER!

Request your free copy
"Built for perfect migration"
from your local distributor!
Unstained
Protein Standard,
Broad Range
10-200 kDa 10-20% Tris-glycine SDS-PAGE
Blue Prestained
Protein Standard,
Broad Range
11-190 kDa 10-20% Tris-glycine SDS-PAGE
Color Prestained
Protein Standard,
Broad Range
11-245 kDa 10-20% Tris-glycine SDS-PAGE

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FREE SAMPLE AVAILABLE
Request your free sample from your local distributor (limited to 1 sample per workgroup. As long as stocks last.)
Color Prestained Protein Standard,
Broad Range, #P7712