NEBNext Ultra II FS DNA Library Prep Kit for Illumina
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NEW PRODUCT:
NEBNEXT® ULTRA™ II FS DNA LIBRARY PREP KIT – NOVEL ENZYMATIC FRAGMENTATION SYSTEM |
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Do you need a faster, more reliable solution for DNA fragmentation and library construction? The NEBNext Ultra II FS DNA Library Prep Kit meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Enzymatic shearing increases library yields by reducing DNA damage and sample loss. Our new fragmentation reagent is also combined with end repair and dA-tailing reagents, and a single protocol is used for the full range of input amounts (100 pg - 500 ng) and GC
content. You'll be thrilled to pieces with the result – a reliable, flexible, high–quality library prep that is fast and scalable. |
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| Libraries were prepared from Human NA19240 genomic DNA using the input amounts and numbers of PCR cycles shown. For NEBNext Ultra II FS, a 20–minute fragmentation time was used. For Kapa HyperPlus libraries, input DNA was cleaned up with 3X beads prior to library construction, as recommended, and a 20–minute fragmentation time was used. Illumina recommends 50 ng input for Nextera, and not an input range; therefore, only 50 ng was used in this experiment. "Covaris" libraries were prepared by shearing each input amount in 1X TE Buffer to an insert size of ~200 bp using a Covaris instrument, followed by library construction using the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645). Error bars indicate standard deviation for an average of 3–6 replicates performed by 2 independent users. | |||
 
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